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| goat anti human IgG(Fc-specific), affinity purified, MinX, Bo Ho Ms - POD conjugate |
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Product Data |
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Product Name |
goat anti human IgG(Fc-specific), affinity purified, MinX, Bo Ho Ms - POD conjugate |
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Catalog No |
SQ-AK-21901 |
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Size |
1 ml |
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Price |
175,00 EUR |
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Category |
Antibodies and Conjugates |
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Description |
affinity purified polyclonal goat antibody, specific to human IgG (Fc), minimal cross reaction to other serum proteins, conjugated to highly active POD |
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Feature |
highly specific polyclonal antibody to human IgG (Fc) |
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Reconstitution |
lyophilized |
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Stabilizer |
reconstitute with 1 ml of PBS WITHOUT sodium azide |
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Storage |
1% BSA without IgG |
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Conjugate/Tag/Label |
-20°C |
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Conjugation Ratio |
POD |
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Protein Source |
1: 1-3 (pAk : POD) |
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Applications |
horse radish |
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Use |
highly specific secondary antibody conjugate for ELISA, Western blot |
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Isotype |
goat |
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Clone |
polyclonal |
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Specificity |
human Fc-fragment |
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Cross Reactions |
the antibody conjugate is specific for the Fc portion of human IgG heavy chain. |
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Affinity Constant |
There is no cross reactivity with human F(ab)2. the antibody was affinity purified and cross-adsorbed against human IgA, human IgM and human F(ab)2 as well as against serum proteins from bovine, mouse and horse. |
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Purification |
n.d. |
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Concentration |
affinity purification on hIgG(Fc)-HYDRA; adsorption against hIgG F(ab)2-HYDRA, hIgA-HYDRA, hIgM-HYDRA, mouse IgG, serum proteins from mouse, bovine and horse - HYDRA matrix; the pAb was covalently coupled to POD, unconjugated monomers were separated by preparative SEC |
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Purity |
1 mg/ml after reconstitution |
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Activity |
>98% |
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Source |
ELISA: > 1:7000 |
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Considerations for Use |
6 months after reconstitution |
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Additional Info |
Please store the vial after reconstitution at 4-8°C. The product is stable for 1 year at 4-8°C in its stock solution. Working dilutions should always be prepared freshly. For extended storage of the stock solution please add 50% glycerol and freeze the product. |
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Protocol |
please do NOT add sodium azide! |
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