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Product Name |
7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) |
ME047.1 (1 mg)
ME047.2 (5 mg) |
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A high-affinity Fe3+ specific fluorescent indicator/chelator |
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A fluorescent analogue of the natural siderophore Desferrioxiamine B |
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The fluorescence is quenched upon complexation with Fe3+ |
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Quantitative assessment of Fe3+ by selective scavenging of Fe3+ |
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| Assessment of the location and the dynamics of cellular iron uptake |
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Assessment of iron reduction |
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Unique permeation properties across membranes |
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| | Product Information | |
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| | NBD-DFO is the fluorescent analogue of the siderophore desferrioxamine B (DFO), coupled to Nitrobenz-2-oxa-1,3-diazole (NBD). NBD-DFO is a highly-specific Fe3+ hydroxamate incicator/chelator(1-3). Its fluorescence is quenched by Fe3+ ions. The lipophilic siderophore, which demonstrates excellent permeation across membranes was used for detecting iron ions in cell-free systems, for recording the transport of iron into microorganisms and plants, for detecting pathogenic microorganisms and for monitoring Fe3+ mobilization from cells. | |
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| | Product Data | |
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| | chemical name | 7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) | |
| | IUPAC name | N'-{5-[acetyl(hydroxy)amino]pentyl}-N-hydroxy-N-(5-{[4-(hydroxy-
{5-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]pentyl}amino)-4-oxobutanoyl]-
amino}pentyl)butanediamide | |
| | molecular formula | C31H49N9O11 | |
| | molecular weight [g/mol] | 723.78 |
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| | absorption maximum: | λmax(log ε) = 480 nm (4.18), 370 (3.68) | |
| | emission maximum: | λmax 548 nm | |
| | affinity for Fe3+: | log Kapp: 31.0 !! (1:1 complex with Fe3+) | |
| | stability | -20oC, stored dry and protected from light | |
| | appearance | yellow solid | |
| | purity | 97%+ (1H NMR, 500 MHz) | |
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| | Considerations for Use | |
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| | The product is used as a fluorescence marker/indicator or chelator for Fe3+ in biological samples, especially to study iron uptake by plants. The dye is fluorescent only when unferrated and can thus be used as a probe to follow iron removal from the sideophore. 1-5 mM stock solutions of NBD-DFO in DMSO can be prepared and aliquots should kept at -20°C. When stored properly at -20°C, the solutions can be used for at least 2 - 3 months. Attention: avoid any contact with metallic surfaces! | |
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| | References | |
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| | 1. S. D. Lytton et. al., Anal. Biochem. (1992) Sept. 205(2): 326-333
2. E. Bar-Ness et al., Plant Physiol. (1992) 99, 1329
3. S. D. Lytton et al., Molecular Pharmacology (1991), 40, 584-590
4. O. Ardon, J. et. al., Bacteriology, | |
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| | Ordering Information | |
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Cat. No. |
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Product Name |
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Quantity |
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Price |
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ME047.1 |
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7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) |
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1 mg |
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325,- € |
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ME047.2 |
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7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) |
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5 mg |
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975,- € |
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* For bulk package size please inquire. Prices do not contain shipping costs, please inquire. |
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