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  Product Name 7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) ME047.1 (1 mg)
ME047.2 (5 mg)
 
 
 graphch/ME047.gif  
 
  A high-affinity Fe3+ specific fluorescent indicator/chelator  
  A fluorescent analogue of the natural siderophore Desferrioxiamine B  
  The fluorescence is quenched upon complexation with Fe3+  
  Quantitative assessment of Fe3+ by selective scavenging of Fe3+  
  Assessment of the location and the dynamics of cellular iron uptake  
  Assessment of iron reduction  
  Unique permeation properties across membranes  
 
 Product Information 
 
 NBD-DFO is the fluorescent analogue of the siderophore desferrioxamine B (DFO), coupled to Nitrobenz-2-oxa-1,3-diazole (NBD). NBD-DFO is a highly-specific Fe3+ hydroxamate incicator/chelator(1-3). Its fluorescence is quenched by Fe3+ ions. The lipophilic siderophore, which demonstrates excellent permeation across membranes was used for detecting iron ions in cell-free systems, for recording the transport of iron into microorganisms and plants, for detecting pathogenic microorganisms and for monitoring Fe3+ mobilization from cells. 
 
 
 Product Data 
 
 chemical name7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO) 
 IUPAC nameN'-{5-[acetyl(hydroxy)amino]pentyl}-N-hydroxy-N-(5-{[4-(hydroxy-
{5-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]pentyl}amino)-4-oxobutanoyl]-
amino}pentyl)butanediamide
 
 molecular formulaC31H49N9O11 
 molecular weight [g/mol]723.78  
 absorption maximum:λmax(log ε) = 480 nm (4.18), 370 (3.68) 
 emission maximum:λmax 548 nm 
 affinity for Fe3+:log Kapp: 31.0 !! (1:1 complex with Fe3+) 
 stability-20oC, stored dry and protected from light 
 appearanceyellow solid 
 purity97%+ (1H NMR, 500 MHz) 
 
 
 Considerations for Use 
 
 The product is used as a fluorescence marker/indicator or chelator for Fe3+ in biological samples, especially to study iron uptake by plants. The dye is fluorescent only when unferrated and can thus be used as a probe to follow iron removal from the sideophore. 1-5 mM stock solutions of NBD-DFO in DMSO can be prepared and aliquots should kept at -20°C. When stored properly at -20°C, the solutions can be used for at least 2 - 3 months. Attention: avoid any contact with metallic surfaces! 
 
 References 
 
 1. S. D. Lytton et. al., Anal. Biochem. (1992) Sept. 205(2): 326-333
2. E. Bar-Ness et al., Plant Physiol. (1992) 99, 1329
3. S. D. Lytton et al., Molecular Pharmacology (1991), 40, 584-590
4. O. Ardon, J. et. al., Bacteriology,
 
 
 Ordering Information 
 
 
  Cat. No. | Product Name | Quantity | Price  
  ME047.1   7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO)   1 mg   325,- €  
  ME047.2   7-Nitrobenz-2-oxa-1,3-diazole-desferrioxamine (NBD-DFO)   5 mg   975,- €  
    * For bulk package size please inquire. Prices do not contain shipping costs, please inquire.  
 
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