|
|
| |
|
|
|
|
|
|
| |
Product Name |
Rhodamine B-[(phenanthren-9-yl)-aminocarbonyl]-benzylester (RPAC) |
ME046.1 (1 mg)
ME046.2 (5 mg) |
|
| |
| |  |
|
| |
| |
 Iron-insensitive control for the mitochondrial fluorescent iron indicator RPA |
|
| |
Mitochondria specific |
|
| |
Non-toxic |
|
| |
| |
| |
| | Product Data | |
| |
| | function | fluorescent, non iron chelating, "negative" control | |
| | chemical name | Rhodamine B-[(phenanthren-9-yl)-aminocarbonyl]-benzylester (RPAC) | |
| | IUPAC name | 6-(diethylamino)-N,N-diethyl-9-[2-({[4-(phenanthren-9-ylcarbamoyl)-
benzyl]oxy}carbonyl)phenyl]-3H-xanthen-3-iminium bromide | |
| | molecular formula | C50H46BrN3O4 | |
| | molecular weight [g/mol] | 832.8219 (752.9174+79.9045) |
|
| | absorption maximum: | λmax (log ε) = 567 nm | |
| | emission maximum: | λmax 602 nm | |
| | stability | +4oC, stored dry and protect from light | |
| | appearance | purple solid | |
| | purity | 97%+ (1H NMR, 500 MHz) | |
| | in vitro toxicity: | non toxic | |
| |
| | | | Considerations for Use | |
| | | | RPAC is used in the same manner as RPA1. 1-5 mM stock solutions of RPAC in DMSO can be prepared and aliquots should be kept at -20°C. When stored properly at -20°C, the frozen aliquots are stable and can be used for at least 2 - 3 months. | |
| | | | Literature | |
| |
| | 1. Selective determination of mitochondrial chelatable iron in viable cells with a new fluorescent sensor. F. Petrat et. al. Biochem. J. (2002) 362, 137-147
2. Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron; U. Rauen et al. Free Radical Biology & Medicine, Vol. 35, No. 12, pp. 1664-1678, 2003
3. The chelatable iron pool in living cells: A methodically defined quantity. F. Petrat et. al. Biol. Chem., Vol. 383, pp. 489-502, 2002
4. Assessment of chelatable mitochondrial iron by using mitochondrion-selective fluorescent iron indicators with different iron-binding affinities. U. Rauen et al. ChemBioChem 2007, 8, 341-352
5. Oxidative inactivation of mitochondrial Aconitase results in iron and hydrogen peroxide-mediated neurotoxicity in rat primary mesencephalic cultures. David Cantu et. al. PlosOne, September 2009, Vol 4, Issue 9, p 1-9 | |
| |
| | Ordering Information | |
| |
| |
| |
Cat. No. |
| |
Product Name |
| |
Quantity |
| |
Price |
|
| |
ME046.1 |
|
Rhodamine B-[(phenanthren-9-yl)-aminocarbonyl]-benzylester (RPAC) |
|
1 mg |
|
240,- € |
|
| |
ME046.2 |
|
Rhodamine B-[(phenanthren-9-yl)-aminocarbonyl]-benzylester (RPAC) |
|
5 mg |
|
725,- € |
|
| |
|
* For bulk package size please inquire. Prices do not contain shipping costs, please inquire. |
|
| | |
